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dapi staining solution  (Boster Bio)


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    Structured Review

    Boster Bio dapi staining solution
    Dapi Staining Solution, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 760 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining+solution+4/DAPI+(4'%2C6-Diamidino-2-Phenylindole%2C+Dihydrochloride)+for+nucleic+acid+staining/pmc13121200-56-20-35
    Average 96 stars, based on 760 article reviews
    dapi staining solution - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Membrane:

    Article Title: Spatial orientation on manufacturing fidelity and mechanical-transport-biological performances of 3D-printed microlattice structures
    Article Snippet: The research on 3D printing of metallic microlattice structures for biological bone scaffolds has attracted more and more attention.. Still, the mechanism by which 3D printing processes constraints such as manufacturing deviations and pore defects affect the mechanical, transport, and biological properties of microlattice scaffolds is unclear.. This work involved constructing diamond-type microlattice structures with different spatial orientations and forming them using laser powder bed fusion additive manufacturing (LPBF-AM).

    Incubation:

    Article Title: The Function of the Lysophospholipase 1 ( LYPLA1 ) Gene in Fetal Sheep Myoblast Proliferation and Myogenic Differentiation.
    Article Snippet: The primary antibody targets the protein MYH3. followed by three washes with PBS and incubation with the prepared secondary antibody solution (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488), 1:200, Abcam, Cambridge Science Park, Cambridge, UK, REF#ab150077) at 37 ◦C in the dark for one hour. .. Finally, the cells were incubated with DAPI staining solution (Boster Biological Technology, Wuhan, China, Catalog#AR1176) at room temperature in the dark for 3–5 min, rinsed with PBS, and immediately placed under an inverted fluorescence microscope (Nikon, Minato, Tokyo, Japan) for photography. ..

    Article Title: The Function of the Lysophospholipase 1 ( LYPLA1 ) Gene in Fetal Sheep Myoblast Proliferation and Myogenic Differentiation
    Article Snippet: The primary antibody targets the protein MYH3. followed by three washes with PBS and incubation with the prepared secondary antibody solution (Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488), 1:200, Abcam, Cambridge Science Park, Cambridge, UK, REF#ab150077) at 37 °C in the dark for one hour. .. Finally, the cells were incubated with DAPI staining solution (Boster Biological Technology, Wuhan, China, Catalog#AR1176) at room temperature in the dark for 3–5 min, rinsed with PBS, and immediately placed under an inverted fluorescence microscope (Nikon, Minato, Tokyo, Japan) for photography. ..

    Article Title: Acupuncture promotes muscle cells ATP metabolism in ST36 acupoint local exerting effect by activating TRPV1/CaMKII/AMPK/PGC1α signaling pathway
    Article Snippet: .. DAPI, a commonly used blue fluorescent DNA dye that can penetrate cell membranes, was applied by adding DAPI staining solution (AR1176, BOSTER, Wuhan, China) to the slides, which were subsequently incubated at room temperature for 3 min, followed by washing with PBS. .. Anti-fade mounting medium (AR1109, BOSTER, Wuhan, China) was used.

    Staining:

    Article Title: The Function of the Lysophospholipase 1 ( LYPLA1 ) Gene in Fetal Sheep Myoblast Proliferation and Myogenic Differentiation.
    Article Snippet: The primary antibody targets the protein MYH3. followed by three washes with PBS and incubation with the prepared secondary antibody solution (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488), 1:200, Abcam, Cambridge Science Park, Cambridge, UK, REF#ab150077) at 37 ◦C in the dark for one hour. .. Finally, the cells were incubated with DAPI staining solution (Boster Biological Technology, Wuhan, China, Catalog#AR1176) at room temperature in the dark for 3–5 min, rinsed with PBS, and immediately placed under an inverted fluorescence microscope (Nikon, Minato, Tokyo, Japan) for photography. ..

    Article Title: Kaempferol attenuates LPS-induced inflammatory responses in H9c2 cells through involvement of the IL-6/JAK2/STAT3 pathway
    Article Snippet: Lipopolysaccharide (LPS) (Cat# S11060) and Dexamethasone (Dex) (Cat# S17003) were obtained from the Nanjing Jiancheng Bioengineering Institute. .. CY3 Fluorescent Antibody (Cat# BA1032), β-actin (Cat# BM3873), DAPI Staining Solution (Cat# AR1176), BCA Protein Concentration Assay Kit (Cat# AR0146), DAPI Staining Solution (Cat# AR1176), BCA Protein Concentration Measurement Kit (Cat# AR0146) were purchased from BOSTER. .. Phospho-STAT3 antibody (Cat# 9145) was purchased from Cell Signaling Technology (Shanghai) Biological Reagents Co., Ltd. Phospho-JAK2 antibody (Cat# ab32101) was obtained from Abcam.

    Article Title: Indolepropionic acid modulates the immune response in allergic rhinitis through the AKT/CEBPB/IL-10 signaling pathway
    Article Snippet: Subsequently, the samples were incubated with the following secondary antibodies: CY3-conjugated avidin (cat. no. BA1037, 1:200) and DyLight ® 488-conjugated avidin (cat. no. BA1128, 1:200) (both from Boster Biological Technology), for 1 h at room temperature in the dark. .. After staining with DAPI solution (cat. no. AR1176; 1:000; Boster Biological Technology) for 10 min at room temperature and washing with PBS three times (2 min/wash), anti-fluorescence quencher (cat. no. AR0036; Boster Biological Technology) was applied to the slides. ..

    Article Title: The Function of the Lysophospholipase 1 ( LYPLA1 ) Gene in Fetal Sheep Myoblast Proliferation and Myogenic Differentiation
    Article Snippet: The primary antibody targets the protein MYH3. followed by three washes with PBS and incubation with the prepared secondary antibody solution (Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488), 1:200, Abcam, Cambridge Science Park, Cambridge, UK, REF#ab150077) at 37 °C in the dark for one hour. .. Finally, the cells were incubated with DAPI staining solution (Boster Biological Technology, Wuhan, China, Catalog#AR1176) at room temperature in the dark for 3–5 min, rinsed with PBS, and immediately placed under an inverted fluorescence microscope (Nikon, Minato, Tokyo, Japan) for photography. ..

    Article Title: Acupuncture promotes muscle cells ATP metabolism in ST36 acupoint local exerting effect by activating TRPV1/CaMKII/AMPK/PGC1α signaling pathway
    Article Snippet: .. DAPI, a commonly used blue fluorescent DNA dye that can penetrate cell membranes, was applied by adding DAPI staining solution (AR1176, BOSTER, Wuhan, China) to the slides, which were subsequently incubated at room temperature for 3 min, followed by washing with PBS. .. Anti-fade mounting medium (AR1109, BOSTER, Wuhan, China) was used.

    Article Title: Targeting Tumor Necrosis Factor-α Mitigates Glucose Fluctuation-Induced Aortic Valve Fibrosis: Insights From Diabetic Rat Models
    Article Snippet: Furthermore, an improved hematoxylin–eosin (HE) staining kit (G1121, Solarbio Technology Co., Ltd., Shanghai, China) and the modified Movat–Russell pentachrome staining kit (G3700, Solarbio Technology Co., Ltd., Shanghai, China) were purchased from Solarbio, China. .. In addition, the concentrated SABC-POD (Mouse/Rabbit IgG) kit (SA2010, BOSTER Biological Technology Co., Ltd., Wuhan, China), DyLight 488 conjugated AffiniPure goat anti-mouse IgG (H + L) (BA1126, BOSTER Biological Technology Co., Ltd., Wuhan, China), DyLight 594 conjugated AffiniPure goat anti-rabbit IgG (H + L) (BA1142, BOSTER Biological Technology Co., Ltd., Wuhan, China), ethylenediaminetetraacetic acid (EDTA) antigen retrieval solution (AR0023, BOSTER Biological Technology Co., Ltd., Wuhan, China), 4 ′ ,6-diamidino-2-phenylindole (DAPI) staining solution (AR1176, BOSTER Biological Technology Co., Ltd., Wuhan, China), human TNF- α enzyme-linked immunosorbent assay (ELISA) kit (EK0525, BOSTER Biological Technology Co., Ltd., Wuhan, China), and human TGF- β 1 ELISA kit (EK0513, BOSTER Biological Technology Co., Ltd., Wuhan, China) were obtained from Boster, China. .. The human collagen 1 ELISA kit (CSB-EL005716HU, CUSABIO BIOTECH CO., Ltd., Wuhan, China) was obtained from CUSABIO, China.

    Fluorescence:

    Article Title: The Function of the Lysophospholipase 1 ( LYPLA1 ) Gene in Fetal Sheep Myoblast Proliferation and Myogenic Differentiation.
    Article Snippet: The primary antibody targets the protein MYH3. followed by three washes with PBS and incubation with the prepared secondary antibody solution (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488), 1:200, Abcam, Cambridge Science Park, Cambridge, UK, REF#ab150077) at 37 ◦C in the dark for one hour. .. Finally, the cells were incubated with DAPI staining solution (Boster Biological Technology, Wuhan, China, Catalog#AR1176) at room temperature in the dark for 3–5 min, rinsed with PBS, and immediately placed under an inverted fluorescence microscope (Nikon, Minato, Tokyo, Japan) for photography. ..

    Article Title: The Function of the Lysophospholipase 1 ( LYPLA1 ) Gene in Fetal Sheep Myoblast Proliferation and Myogenic Differentiation
    Article Snippet: The primary antibody targets the protein MYH3. followed by three washes with PBS and incubation with the prepared secondary antibody solution (Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488), 1:200, Abcam, Cambridge Science Park, Cambridge, UK, REF#ab150077) at 37 °C in the dark for one hour. .. Finally, the cells were incubated with DAPI staining solution (Boster Biological Technology, Wuhan, China, Catalog#AR1176) at room temperature in the dark for 3–5 min, rinsed with PBS, and immediately placed under an inverted fluorescence microscope (Nikon, Minato, Tokyo, Japan) for photography. ..

    Microscopy:

    Article Title: The Function of the Lysophospholipase 1 ( LYPLA1 ) Gene in Fetal Sheep Myoblast Proliferation and Myogenic Differentiation.
    Article Snippet: The primary antibody targets the protein MYH3. followed by three washes with PBS and incubation with the prepared secondary antibody solution (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488), 1:200, Abcam, Cambridge Science Park, Cambridge, UK, REF#ab150077) at 37 ◦C in the dark for one hour. .. Finally, the cells were incubated with DAPI staining solution (Boster Biological Technology, Wuhan, China, Catalog#AR1176) at room temperature in the dark for 3–5 min, rinsed with PBS, and immediately placed under an inverted fluorescence microscope (Nikon, Minato, Tokyo, Japan) for photography. ..

    Article Title: The Function of the Lysophospholipase 1 ( LYPLA1 ) Gene in Fetal Sheep Myoblast Proliferation and Myogenic Differentiation
    Article Snippet: The primary antibody targets the protein MYH3. followed by three washes with PBS and incubation with the prepared secondary antibody solution (Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488), 1:200, Abcam, Cambridge Science Park, Cambridge, UK, REF#ab150077) at 37 °C in the dark for one hour. .. Finally, the cells were incubated with DAPI staining solution (Boster Biological Technology, Wuhan, China, Catalog#AR1176) at room temperature in the dark for 3–5 min, rinsed with PBS, and immediately placed under an inverted fluorescence microscope (Nikon, Minato, Tokyo, Japan) for photography. ..

    Protein Concentration:

    Article Title: Kaempferol attenuates LPS-induced inflammatory responses in H9c2 cells through involvement of the IL-6/JAK2/STAT3 pathway
    Article Snippet: Lipopolysaccharide (LPS) (Cat# S11060) and Dexamethasone (Dex) (Cat# S17003) were obtained from the Nanjing Jiancheng Bioengineering Institute. .. CY3 Fluorescent Antibody (Cat# BA1032), β-actin (Cat# BM3873), DAPI Staining Solution (Cat# AR1176), BCA Protein Concentration Assay Kit (Cat# AR0146), DAPI Staining Solution (Cat# AR1176), BCA Protein Concentration Measurement Kit (Cat# AR0146) were purchased from BOSTER. .. Phospho-STAT3 antibody (Cat# 9145) was purchased from Cell Signaling Technology (Shanghai) Biological Reagents Co., Ltd. Phospho-JAK2 antibody (Cat# ab32101) was obtained from Abcam.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Targeting Tumor Necrosis Factor-α Mitigates Glucose Fluctuation-Induced Aortic Valve Fibrosis: Insights From Diabetic Rat Models
    Article Snippet: Furthermore, an improved hematoxylin–eosin (HE) staining kit (G1121, Solarbio Technology Co., Ltd., Shanghai, China) and the modified Movat–Russell pentachrome staining kit (G3700, Solarbio Technology Co., Ltd., Shanghai, China) were purchased from Solarbio, China. .. In addition, the concentrated SABC-POD (Mouse/Rabbit IgG) kit (SA2010, BOSTER Biological Technology Co., Ltd., Wuhan, China), DyLight 488 conjugated AffiniPure goat anti-mouse IgG (H + L) (BA1126, BOSTER Biological Technology Co., Ltd., Wuhan, China), DyLight 594 conjugated AffiniPure goat anti-rabbit IgG (H + L) (BA1142, BOSTER Biological Technology Co., Ltd., Wuhan, China), ethylenediaminetetraacetic acid (EDTA) antigen retrieval solution (AR0023, BOSTER Biological Technology Co., Ltd., Wuhan, China), 4 ′ ,6-diamidino-2-phenylindole (DAPI) staining solution (AR1176, BOSTER Biological Technology Co., Ltd., Wuhan, China), human TNF- α enzyme-linked immunosorbent assay (ELISA) kit (EK0525, BOSTER Biological Technology Co., Ltd., Wuhan, China), and human TGF- β 1 ELISA kit (EK0513, BOSTER Biological Technology Co., Ltd., Wuhan, China) were obtained from Boster, China. .. The human collagen 1 ELISA kit (CSB-EL005716HU, CUSABIO BIOTECH CO., Ltd., Wuhan, China) was obtained from CUSABIO, China.

    other:

    Article Title: Acupuncture promotes muscle cells ATP metabolism in ST36 acupoint local exerting effect by activating TRPV1/CaMKII/AMPK/PGC1α signaling pathway.
    Article Snippet: For fluorescent secondary antibody incubation, a mixture of Alexa Fluor 488-labeled goat anti-rabbit IgG (Ab150077, Abcam, 1:500, USA) and Alexa Fluor 647-labeled goat anti-mouse IgG (Ab150115, Abcam, 1:500, USA) was added to the tissues and incubated at 37 °C for 45 min.



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    Colocalization of CD3, CD68, and JAK1 in the aortic valves of diabetic rat models . (A,B) The coimmunostaining analysis of the HG and GF groups demonstrated the presence of CD3, CD68, and JAK1 proteins, indicating that JAK1 expression is primarily localized in T lymphocytes and macrophages. Notably, this expression pattern could be altered through the inhibition of TNF- α . Scale bar = 50 µm. DAPI, 4 ′ <t>,6-diamidino-2-phenylindole.</t>
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    Image Search Results


    Colocalization of CD3, CD68, and JAK1 in the aortic valves of diabetic rat models . (A,B) The coimmunostaining analysis of the HG and GF groups demonstrated the presence of CD3, CD68, and JAK1 proteins, indicating that JAK1 expression is primarily localized in T lymphocytes and macrophages. Notably, this expression pattern could be altered through the inhibition of TNF- α . Scale bar = 50 µm. DAPI, 4 ′ ,6-diamidino-2-phenylindole.

    Journal: Reviews in Cardiovascular Medicine

    Article Title: Targeting Tumor Necrosis Factor-α Mitigates Glucose Fluctuation-Induced Aortic Valve Fibrosis: Insights From Diabetic Rat Models

    doi: 10.31083/RCM42804

    Figure Lengend Snippet: Colocalization of CD3, CD68, and JAK1 in the aortic valves of diabetic rat models . (A,B) The coimmunostaining analysis of the HG and GF groups demonstrated the presence of CD3, CD68, and JAK1 proteins, indicating that JAK1 expression is primarily localized in T lymphocytes and macrophages. Notably, this expression pattern could be altered through the inhibition of TNF- α . Scale bar = 50 µm. DAPI, 4 ′ ,6-diamidino-2-phenylindole.

    Article Snippet: In addition, the concentrated SABC-POD (Mouse/Rabbit IgG) kit (SA2010, BOSTER Biological Technology Co., Ltd., Wuhan, China), DyLight 488 conjugated AffiniPure goat anti-mouse IgG (H + L) (BA1126, BOSTER Biological Technology Co., Ltd., Wuhan, China), DyLight 594 conjugated AffiniPure goat anti-rabbit IgG (H + L) (BA1142, BOSTER Biological Technology Co., Ltd., Wuhan, China), ethylenediaminetetraacetic acid (EDTA) antigen retrieval solution (AR0023, BOSTER Biological Technology Co., Ltd., Wuhan, China), 4 ′ ,6-diamidino-2-phenylindole (DAPI) staining solution (AR1176, BOSTER Biological Technology Co., Ltd., Wuhan, China), human TNF- α enzyme-linked immunosorbent assay (ELISA) kit (EK0525, BOSTER Biological Technology Co., Ltd., Wuhan, China), and human TGF- β 1 ELISA kit (EK0513, BOSTER Biological Technology Co., Ltd., Wuhan, China) were obtained from Boster, China.

    Techniques: Expressing, Inhibition